当前位置:首页 > 研究工作 > 发表论文
发表论文

Single-cell transcriptome analysis reveals cell lineage specification in temporal-spatial patterns in human cortical development, Sci Adv, 21 Aug 2020

发表日期:2020-08-21 08:20  作者:

Science Advances, 21 August, 2020, DOI:https://doi.org/10.1126/sciadv.aaz2978

Single-cell transcriptome analysis reveals cell lineage specification in temporal-spatial patterns in human cortical development

Xiaoying Fan#, Yuanyuan Fu#, Xin Zhou#, Le Sun#, Ming Yang, Mengdi Wang, Ruiguo Chen, Qian Wu, Jun Yong, Ji Dong, Lu Wen, Jie Qiao*, Xiaoqun Wang* and Fuchou Tang*

Abstract

Neurogenesis processes differ in different areas of the cortex in many species, including humans. Here, we performed single-cell transcriptome profiling of the four cortical lobes and pons during human embryonic and fetal development. We identified distinct subtypes of neural progenitor cells (NPCs) and their molecular signatures, including a group of previously unidentified transient NPCs. We specified the neurogenesis path and molecular regulations of the human deep-layer, upper-layer, and mature neurons. Neurons showed clear spatial and temporal distinctions, while glial cells of different origins showed development patterns similar to those of mice, and we captured the developmental trajectory of oligodendrocyte lineage cells until the human mid-fetal stage. Additionally, we verified region-specific characteristics of neurons in the cortex, including their distinct electrophysiological features. With systematic single-cell analysis, we decoded human neuronal development in temporal and spatial dimensions from GW7 to GW28, offering deeper insights into the molecular regulations underlying human neurogenesis and cortical development.

文章链接:https://advances.sciencemag.org/content/6/34/eaaz2978

相关报道:http://www.ibp.cas.cn/kyjz/zxdt/202008/t20200824_5671695.html

 

 

联系我们 | 实验室方位
Copyright © 2007-2016 脑与认知科学国家重点实验室 备案序号:京ICP备000000000号
地址:北京市朝阳区大屯路15号 100101 电话:010 64861049