报告题目：Rapid functional imaging of neurons

报告时间：2018 年 4 月 23 日，上午 10:00-12:00， 6937 会议室

报告人 ：Rongwen  Lu,    Janelia  Research  Campus,  Howard  Hughes Medical Institute.

主持人：刘力研究员 

Abstract: Functional monitoring of individual neurons and neural circuits, e.g., either in the retina or the brain, is crucial to understand neuronal mechanisms. There are two desirable goals for functional imaging of neurons: cellular or even synaptic spatial resolution and rapid imaging speed to resolve individual neuronal activities. In this talk, I will introduce line illuminations, either in the lateral or axial direction, to enhance the imaging speed while maintaining the spatial resolutions. The lateral line illumination was incorporated into the optical coherence tomography for rapid intrinsic optical imaging of retinal photoreceptors; while the axial line illumination, a.k.a. Bessel beam, was integrated into the two-photon microscope for video-rate volumetric functional imaging of neurons in animal brains. Our approach represents a powerful new technology to study functions of neurons either in the retina or the brain.

Biography: Rongwen Lu currently is a postdoc in Dr. Na Ji’s lab at Janelia Research Campus, Howard Hughes Medical Institute. He has had a strong interest in physics, especially in optics, since high school, which motivated him to study Optical Engineering and obtained the B.S degree from University of Shanghai for Science and Technology in China. After that, Rongwen went to graduate schools in the United States. He got a M.S. in Vision Science from Indiana University at Bloomington in 2010 supervised by Professor Ann Elsner and a Ph.D. in Biomedical Engineering from Alabama University at Birmingham in 2014 under the guidance of Professor Xincheng Yao. During his M.S and Ph.D., he mainly focused on developing novel and clinically useful optical imaging techniques for early diagnosis of retinal diseases. In his postdoc work, he applied novel optical techniques in neuroscience and has devised a Bessel beam module that can tremendously enhance the volume rate of two-photon microscope for brain imaging.